The "Anatomy" subsection is divided into four areas: "External Anatomy", "Internal Anatomy", "Skull and Skeleton" , and "Mistaken Identity".

"Reproduction and Development" describes what is known of the thylacine's reproductive biology and early developmental stages, through historical accounts as well as examination of preserved specimens in museum collections.

In "Behaviour" historical observations that were made of the thylacine in both the wild and captivity, bringing together an account of the species's dietary habits, hunting methods, vocalisations and other behavioural aspects.

"The Specimens" subsection provides an account of all the museum specimens that are known to physically remain of this unique species.