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BIOLOGY:
- THE SPECIMENS -
(page 9)
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Pouch Young:

    There are a total of twelve alcohol preserved pouch young listed within the 4th revision of the International Thylacine Specimen Database (2011).  Two of these specimens, registered in the catalogue of the Museum of Vertebrates at Cornell University as "foetal specimens" [CU75 & CU77], could not be located (17/5/2010) and are now presumed to no longer exist.  Of the ten remaining pouch young, a male specimen in the collection of the National Museum of Victoria [NMV C5754] was sectioned for microscopy in 1994 by Professor Milan Klima of the Institute of Anatomy, Johann Wolfgang Goethe University, Frankfurt, and remains in the museum's collection as a series of microscopy slides.

specimen C5754
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Specimen C5754 [now sectioned]. Courtesy: Museum Victoria. Photo: Prof. Dr. Heinz Moeller.  Source: International Thylacine Specimen Database (2013).
thylacine pouch young specimens C5755 - C5757
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Thylacine pouch young specimens C5755 - C5757. Courtesy: Museum Victoria.  Source: International Thylacine Specimen Database (2013).

    Of the nine whole specimens, three are in the collection of the National Museum of Victoria [NMV] in Melbourne [C5755, C5756, C5757], one in the Australian Museum [AMS] in Sydney [P762], and five in the Tasmanian Museum & Art Gallery [TMAG] in Hobart [A930, A931, A932, A933, A934].  Four of these specimens are males, four are females, with one [TMAG A933], where the sex is yet to be determined.

thylacine pup specimens TMAG A930, 931, 932, 933 & 934
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Thylacine pup specimens TMAG A930, 931, 932, 933 & 934.  Courtesy: Tasmanian Museum & Art Gallery.
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    The four NMV pouch young together with their mother were obtained as dead specimens from William M. McGowan, Superintendant of the Launceston City Park Zoo (1882-1937), on the 23rd June 1909.
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The AMS pouch specimen was obtained on a collecting trip to Tasmania in 1866 by George Masters, the museum's assistant curator (1864-1874).  In a letter addressed to the Australian Museum dated 27th December 1866, Masters states:

    "I have shipped today per City of Hobart a large case containing a black fish and a young thylacine (in spirit); these I have obtained from the museum in exchange for birds, shells and another small box containing 2893 insects".

    The TMAG pouch young were collected from different sources during the 1920s and early 1930s by Dr Joseph Pearson, the Director of the museum from 1934 until 1952.

    The external morphology of thylacine pouch young was first described by Boardman (1945) in his paper, "Some points in the external morphology of the pouch young of the marsupial, Thylacinus Cynocephalus".  Boardman presents a comparison of the external features (hair, pigmentation, hair tracks, facial vibrissae, lips and oral fissure, rhinarium, eye, external ear, feet and marsupium) of the pouch specimen in the Australian Museum collection [P762] with the pouch specimens (litter of 4) in the National Museum Victoria collection [C5754, C5755, C5756, C5757].

specimen AMS P762
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Specimen AMS P762. Courtesy: Australian Museum. Photo: Prof. Dr. Heinz Moeller.  Source: International Thylacine Specimen Database (2013).
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    Without doubt, the single most important discovery in the 5th revision of the ITSD (2013) was the discovery in November 2011 of a litter of four pouch young (DZCU 8021[1-4]) in the collection of the Department of Zoology (Faculty of Science) at Charles University in Prague.  The pouch young were described for the first time in a paper by Sleightholme, Robovský & Vohralík published in the Australian Zoologist in December 2012.  These specimens are the only known wet pouch young outside of Australia, and the earliest in terms of their ontogeny (development); with an estimated age of < 2 weeks.
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pouch young specimens DZCU 8021 [1-4]
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Left: Pouch young specimens DZCU 8021 [1-4].  Photo: Jan Robovský.
Right: Specimen DZCU 8021 [1].  Photo: Tereza Holicová.
Source: Sleightholme, Robovský & Vohralík, Australian Zoologist (2012).
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    The discovery of the Charles University specimens takes the total of wet pouch young specimens in collections to 14, one of which exists as a series of microscopy sections (this total excludes the two Cornell University specimens [CU75 & CU77] that cannot be located).  The Charles University litter is unique in providing researchers with a valuable insight into the early development of the thylacine.
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References
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